The Development of a Point of Care Creatinine Measurement Using Disposable Ready to Use Microchip Capillary Electrophoresis
نویسندگان
چکیده
We report on the determination of creatinine in human serum using a point-of-care device suitable for the use by untrained operators, the Medimate Multireader®. This device is based on electrophoretic separation and conductivity detection and its prefilled microfluidic chip has a single opening for sample introduction. The device was originally developed for the determination of lithium in blood [1]. Creatinine determination at a level of quantification of 300 μM was realized by optimizing the background electrolyte (BGE) pH and concentration, and the injection and separation potentials and time. This limit of quantification is in the relevant range for the detection of renal failure [2].
منابع مشابه
Improved Precision Using Internal Standards in Disposable Point-of-care Capillary Electrophoresis Devices
This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using inhouse fabricated microchips. Five concentrations of NaCl were analyzed consecuti...
متن کاملIntegrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification
To improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-chip variabilities inherent in disposable, single-use devices must be addressed. This work proposes to integrate an internal standard (ISTD) into the microchip by adding it to the background electrolyte (BGE) instead of the sample-thus eliminating the need for additional sample manipulation, mic...
متن کاملMicrochip capillary electrophoresis for point-of-care analysis of lithium.
BACKGROUND Microchip capillary electrophoresis (CE) is a promising method for chemical analysis of complex samples such as whole blood. We evaluated the method for point-of-care testing of lithium. METHODS Chemical separation was performed on standard glass microchip CE devices with a conductivity detector as described in previous work. Here we demonstrate a new sample-to-chip interface. Init...
متن کاملQuantitative Microchip Capillary Electrophoresis for Inorganic Ion Analysis at the Point of Care
No part of this work may be reproduced by print, photocopy or any other means without the permission in writing from the author. 1.2 Microchip capillary electrophoresis of inorganic ions for point-of-care analysis ..6
متن کاملFabrication of microfluidic devices using dry film photoresist for microchip capillary electrophoresis.
An inexpensive, disposable microfluidic device was fabricated from a dry film photoresist using a combination of photolithographic and hot roll lamination techniques. A microfluidic flow pattern was prefabricated in a dry film photoresist tape using traditional photolithographic methods. This tape became bonded to a poly(methyl methacrylate) (PMMA) sheet with prepouched holes when passed throug...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011